R&D systems Antibody Technical Tip

 

 

Q1: Epitope¿¡ ´ëÇÑ Á¤º¸(A.A, Á¤È®ÇÑ ºÎºÐ µî)À» ¾Ë ¼ö ÀÖÀ»±î¿ä?

 

A: Monoclonal antibody¿Í polyclonal antibody°¡ ÀνÄÇÏ´Â Á¤È®ÇÑ epitope ºÎºÐ¿¡ ´ëÇÑ epitope

map Á¤º¸¸¦ °¡Áö°í ÀÖÁö ¾Ê½À´Ï´Ù. Data sheet»ó¿¡ ¾ð±ÞµÈ entire Immunogen (e.g. Accession # Q60805)À» »ç¿ëÇÏ¿© AntibodyÀ» ¸¸µì´Ï´Ù. ¸¸¾à peptide immunogenÀÌ »ç¿ëµÇ¾úÀ» °æ¿ì, data sheet¿¡¼­ È®ÀÎ ÇÒ ¼ö ÀÖ½À´Ï´Ù.

 

 

Q2: Data sheet¿¡ ¾ø´Â applicationÀÎ °æ¿ì¿¡µµ »ç¿ë °¡´ÉÇÑ°¡¿ä?

 

A: ƯÁ¤ applicationÀÌ datasheet»ó¿¡¼­ È®ÀÎÀÌ ¾È µÇ°í, R&D systems¿¡¼­ in-house test dataÀ» °¡Áö°í ÀÖÁö ¾ÊÀº °æ¿ì¿¡ Technical Service¿¡¼­´Â reference databaseÀ» ÅëÇÏ¿© ºñ½ÁÇÑ Àû¿ë »ç·Ê¸¦ È®ÀÎÇØ µå¸± ¼ö´Â ÀÖÀ¸³ª, guarantee µÇÁö ¾Ê½À´Ï´Ù.

 

 

Q3: R&D systems AntibodiesÀÇ expirationÀº ¾ðÁ¦Àΰ¡¿ä?

 

A: À̷лó ¹Ì °³ºÀ»óÅ¿¡¼­ freeze/thaw cycleÀ» ¹Ýº¹ÇÏÁö ¾ÊÀº lyophilized powder »óÅ·Π-20 ~-80 °C¿¡¼­ 0.1mg/mlÀÌ»óÀÇ ³óµµ·Î º¸°üµÉ °æ¿ì, Àϳâ ÀÌ»ó º¸°üµµ °¡´ÉÇϳª »ç¿ëÀÚÀÇ º¸°ü »óÅ ¹× Á¦Ç°¿¡ µû¶ó º¸°ü ¹æ¹ý°ú ±â°£ÀÌ ´Ù¸¦ ¼ö ÀÖ½À´Ï´Ù. R&D systems¿¡¼­´Â ProteinÀÇ activity¿¡ ´ëÇÑ warranty°¡ °¡´ÉÇÑ ÀϱîÁö¸¦ expiration date·Î Á¤ÇÏ°í ÀÖ½À´Ï´Ù. ÀϹÝÀûÀ¸·Î ±¸¸ÅÀϷκÎÅÍ 6~12°³¿ùÀ̳ª Á¦Ç°¸¶´Ù ´Ù¸¦ ¼ö ÀÖÀ¸´Ï ²À, product insertÀ» È®ÀÎÇÏ°í ±ÇÀå º¸°ü Á¶°ÇÀ» ÁؼöÇÏ¿© ÁֽʽÿÀ.

 

 

Q4: Formulation¿¡ Æ÷ÇԵǾî ÀÖ´Â trehalose´Â ¾î¶² ¹°ÁúÀΰ¡¿ä?

 

A: Trehalose´Â non-reducing sugar(MW342.1)·Î amino acids³ª proteins¿¡ ¹ÝÀÀÇÏÁö ¾Ê½À´Ï´Ù. ÀÌ´Â ½Ä¹°À̳ª µ¿¹°¿¡¼­ ¹ß°ßµÇ´Â ÀÚ¿¬¹°Áú·Î, freezing¿¡ ÀÇÇÑ ¼Õ»óÀ» ÃÖ¼ÒÈ­ÇÏ´Â °¡Àå È¿°úÀûÀÎ sugarÁß¿¡ Çϳª ÀÔ´Ï´Ù. µ¿°á °ÇÁ¶½Ã proteins ¼öºÐ Èí¼ö¿¡ ´ëÇÑ ÀúÇ×¼ºÀ» ³ô¿©ÁÖ¾î, ±× °á°ú reconstitution ½Ã ħÀüµÇÁö ¾Ê°Ô µË´Ï´Ù.

 

 

Q5: Trehalose°¡ ½ÇÇè(conjugation, animal injection, other applications)¿¡ ¿µÇâÀ» ÁÖ³ª¿ä?

 

A: °¡´ÉÇÑ ¸ðµç applications¿¡ ¾î¶² ¿µÇâÀ» ÁÙÁö´Â ¸ð¸£Áö¸¸, R&D systems¿¡¼­ ½Ç½ÃÇÑ Bioassays³ª ¾Ë·ÁÁø applications¿¡¼­´Â ºÎÁ¤ÀûÀÎ ¿µÇâÀ» º¸ÀÌÁö ¾Ê¾Ò½À´Ï´Ù. Á¦Ç° ³» trehaloseÀÇ ³óµµ°¡ ºÎÁ¤ÀûÀÎ ¿µÇâÀ» º¸ÀÌ´ÂÁö ¾Æ´ÑÁö º»ÀÎÀÇ ½ÇÇè¿¡¼­ trehalose¸¦ control·Î »ç¿ëÇÏ¿© È®ÀÎÇϽñæ Ãßõ µå¸³´Ï´Ù.

 

 

Q6: IgGÀÇ molecular weightÀÌ ¾ó¸¶Àΰ¡¿ä?

 

A: IgG proteinÀº µÎ °³ÀÇ heavy chain (¾à 55kDa)°ú µÎ °³ÀÇ light chains(¾à 25kDa)À¸·Î ±¸¼ºµÇ¾î ÀÖ½À´Ï´Ù. µû¶ó¼­ ÃÑ ¾à 160kDa ÀÔ´Ï´Ù.

 

 

Q7: Donkey·ÎºÎÅÍ ¾òÀº absÀ» ±¸¸ÅÇϴµ¥ Á¦ÇÑÀÌ ÀÖ³ª¿ä?

 

A: the Convention on International Trade in Endangered Species of Wild Fauna and Flora(CITES)¿¡ ÀÇÇØ Á¦ÇѵÇÁö ¾ÊÀ¸¸ç, CITES permitÀÌ ¿ä±¸µÇ´Â Á¦Ç°ÀÌ ¾Æ´Õ´Ï´Ù.

 

 

Q8: ReconstitutionÇÑ antibodyÀÇ ³óµµ¸¦ ÃøÁ¤ÇÏ°í ½Í¾î¿ä.

 

A: antibody´Â ÀϹÝÀûÀ¸·Î spectrophotometry¸¦ ÀÌ¿ëÇؼ­ A280 Èí±¤µµ¸¦ ÃøÁ¤ÇÕ´Ï´Ù. ÀÌ ¶§ ³ª¿Â °ªÀ» IgG Èí±¤°è¼öÀÎ 1.4·Î ³ª´©¾î¼­ ³ª¿Â °ªÀÌ ÇØ´ç antibodyÀÇ ³óµµ°¡ µË´Ï´Ù. (´ÜÀ§: mg/ml) ¸¸ÀÏ antibodyÀÇ ³óµµ¸¦ Bradford assay¸¦ ÀÌ¿ëÇؼ­ ÃøÁ¤ÇÏ°í ½Í´Ù¸é, controlÀ» IgG·Î ÀâÀº ÈÄ ÃøÁ¤ÇØ¾ß ´õ Á¤È®ÇÑ °ªÀ» ¾òÀ¸½Ç ¼ö ÀÖ½À´Ï´Ù.

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