2014. 06. 11.                                                                                                                

 

    Cell Viability Assay..

          CCK-8°ú ÇÔ²² ¿©À¯¸¦ ´©¸®¼¼¿ä.

 

CCK-8 vs MTT ºñ±³ µ¿¿µ»ó

 

CCK-8 ¸¸ÀÇ Æ¯ÀåÁ¡

      • MTT, XTT, MTS, WST-1 ´ëºñ 2~3¹è ¶Ù¾î³­ °¨µµ
      • 24½Ã°£ incubation¿¡µµ ¼¼Æ÷°¡ Á×Áö ¾Ê´Â ¹«µ¶¼º
      • ³ÃÀå°í¿¡¼­ ²¨³» ¹Ù·Î ¾²´Â Æí¸®¼º
      • Long Stability (³ÃÀå 1³â)
      • Suspension Cell ¿¡¼­µµ ¿ì¼öÇÑ ÀçÇö¼º
      • ºû¿¡ ÀÇÇÑ º¯¼ºÀÌ °ÅÀÇ ¾ø´Â ¾ÈÁ¤¼º
      • ¹æ»ç¼º µ¿À§¿ø¼Ò, DMSO ºÒÇÊ¿ä
      • Harvest, Washing, Solubilization °úÁ¤ ºÒÇÊ¿ä.

 

 

  ³ÃÀå°í¿¡¼­ ²¨³»¼­ ¹Ù·Î ³Ö°í ÂïÀ¸¸é ³¡!!! CCK-8Àº preparation

  °úÁ¤°ú dissolve °úÁ¤ÀÌ ÇÊ¿ä ¾ø½À´Ï´Ù. Æí¸®ÇÔµµ ÀåÁ¡ÀÌÁö¸¸

  Suspersion cell¿¡µµ Àû¿ë °¡´ÉÇÏ´Ù´Â Å« ÀåÁ¡ÀÌ ÀÖ½À´Ï´Ù.

CCK-8

MTS

MTT

  ¼¼Æ÷¿Í ½ÇÇèÀÚ ¸ðµÎ¿¡°Ô ¾ÈÀüÇÑ Á¦Ç°!! MTS¿Í MTT´Â ¹ÝÀÀ ÈÄ

  ºÒ°ú 3½Ã°£ ¸¸¿¡ ¼¼Æ÷µéÀÌ »ç¸êµÇÁö¸¸, CCK-8Àº ¹«·Á 24½Ã°£

  ¹ÝÀÀ¿¡µµ ¼¼Æ÷µéÀÌ °Ç°­ÇÏ°Ô À¯ÁöµË´Ï´Ù.

 

 

  SensitivityÀÇ È®¿¬ÇÑ Â÷ÀÌ°¡ º¸À̽óª¿ä? MTT¿¡¼­ ½ÇÇ豺°ú

  ´ëÁ¶±º »çÀÌ¿¡ Â÷ÀÌ°¡ º¸ÀÌÁö ¾Ê´Â´Ù¸é MTTÀÇ ³·Àº sensitivity

  ¶§¹®ÀÏ ¼ö ÀÖ½À´Ï´Ù. ¹«·á »ùÇ÷ΠÅ×½ºÆ® Çغ¸¼¼¿ä (¾Æ·¡ ÂüÁ¶)

  ÇÊ¿äÇÒ¶§ ¸¶´Ù ³ìÀÌ°í.. ¼¯°í.. ºÒÆíÇÏÁö ¾ÊÀ¸¼¼¿ä? ¶Ç, ³²Àº ½Ã¾à

  ÀÇ È°¼ºÀÌ ¶³¾îÁöÁö´Â ¾Ê¾ÒÀ»Áö ºÒ¾ÈÇÏÁö ¾ÊÀ¸¼¼¿ä? CCk-8Àº

  ³ÃÀ庸°üÀ¸·Î 1³â µ¿¾ÈÀ̳ª È°¼ºÀÌ À¯ÁöµË´Ï´Ù.

 

 

CCK-8 ¹«·á »ùÇàÁ¦°ø¾È³»

 CCK-8À» ½áº¸°í´Â ½ÍÀºµ¥, Ȥ½Ã¶óµµ ½ÇÇè ȯ°æ¿¡ ¸ÂÁö ¾ÊÀ»±îºÁ ¼±¶æ ±¸ÀÔÀÌ ¸Á¼³¿©

 Áö½ÃÁÒ? ÀúÈñ ¿õºñ ¸ÞµðÅØ¿¡¼­´Â ¼±»ý´ÔµéÀÇ ±×·± ºÒ¾ÈÀ» ´ú¾îµå¸®°íÀÚ CCK-8 »ùÇÃÀ»

 ¹«·á·Î Á¦°øÇØ µå¸®°í ÀÖ½À´Ï´Ù. 100 test ºÐ·®À¸·Î 96-well plate ÇÑÆÇÀ» ÃæºÐÈ÷ ÇÏ°íµµ

 ³²´Â ºÐ·®ÀÔ´Ï´Ù. ±¸ÀÔ Àü¿¡ ¹Ì¸® ÃæºÐÈ÷ Å×½ºÆ® ÇØ º¸½Ã°í, ¸¸Á·ÇÏ½Ã¸é ±¸¸ÅÇØ ÁÖ¼¼¿ä.

 

 

CCK-8 ¹«·á »ùÇà ½Åû ¹æ¹ý


¾Æ·¡ÀÇ ¾ç½ÄÀ» ´Ù¿î·Îµå ¹ÞÀ¸½Å ÈÄ, ºóÄ­À» ä¿ö ¿õºñ ÇмúÆÀÀ¸·Î E-mailÀ» º¸³»Áֽðųª ÀüÈ­ Áֽøé

3~4ÀÏ ¾È¿¡ Åùè·Î ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù (Àç°í ¼ÒÁø½Ã µû·Î ¿¬¶ô µå¸®°Ú½À´Ï´Ù).

 

»ùÇà ½Åû¼­
´Ù¿î·Îµå

  • CCK-8 Çмú´ã´ç ½ÅÁÖÈñ
  • ÀüÈ­ :031-776-3300 (³»¼± 124)

 

 

°ü·ÃÀÚ·á ´Ù¿î·Îµå

Protocol

 

CCK-8 vs MTT

 

Reference

  CCK-8ÀÇ ÀÛ¿ë ¿ø¸®¿Í

  ½ÇÇè°úÁ¤¿¡ ´ëÇØ ÀÚ¼¼È÷

  ¼³¸íÇÑ ÀÚ·áÀÔ´Ï´Ù.

 

  CCK-8°ú MTT  µÎ ¹æ½ÄÀÇ

 Â÷À̸¦ ¼³¸íÇÑ ÀÚ·áÀÔ´Ï´Ù.

 

  °¢ ¼¼Æ÷ÁÖ¿¡¼­ CCK-8 È°¿ë¹ý

  °ú Ãʱâ½ÇÇè¿¡ ÇÊ¿äÇÑ ¼¼Æ÷¼ö

  ¸¦ Á¤¸®ÇÑ ÀÚ·áÀÔ´Ï´Ù.

 

°ü·ÃÁ¦Ç° ¾È³»

 Microbial Viability Assay Kit
 Cat#
M439-10

  • ¹ÚÅ׸®¾Æ¿ë CCK-8
  • gram +/- , yeast ¿¡¼­ »ç¿ë °¡´É

 Double Staining Kit (1 set)
 Cat# CS01-10

  • live cell°ú dead cellÀ» Çü±¤À¸·Î double staining ÇÏ´Â kit

 LDH Assay Kit-WST
 Cat#
CK12-01 (100T)
 Cat# CK12-05 (500T)

 Cat#
CK12-20 (2000T)

  • Dead cellÀ» ÃøÁ¤ÇÏ´Â CCK-8 kit
  • ADCC, CDC assay Àû¿ë °¡´É

 HilyMax
 Cat#
H357-10 (1 ml)
 Cat# H357-15 (5 ml)

 Cat#
H357-20 (10 ml)

  • Insect Cell¿¡ ÃÖÀûÈ­ µÈ transfection reagent
  • ´Ù¾çÇÑ ¼¼Æ÷ÁÖ¿¡¼­µµ ¿ì¼öÇÑ transfection efficiency.

 Tocriscreen (1120 compound)
 Cat# 2884/1SET

  • ¼¼Æ÷ »ý¸® È°¼ºÀÌ °ËÁõµÈ 1120°¡Áö È­ÇÕ¹°À» ¸ð¾Æ³õÀº compound library
  • Tocris¿¡¼­¸¸ »ý»êµÇ´Â È­ÇÕ¹° »ó´ç¼ö Æ÷ÇÔ
  • Cyto toxicity / proliferation screening¿¡ ÃÖÀûÀÇ ¾ÆÀÌÅÛ

 

 

Á¦Ç° »ó´ã : Çмú¸¶ÄÉÆÃÆÀ ½ÅÁÖÈñ (techserv@woongbee.com/ 031-776-3300 ³»¼±124)