2015. 12. 09

 

CD4+ T Cell Cytokine Secretion

 

 

 CD4+ T cell cytokine, transcription factor ޾ پ subset (Th1, Th2, Th17 )

ȭǸ, B cell ü , macrophage ׹׸ Ȱ , cytotoxic T cell Ȱȭ

鿪 ۿ뿡 ߿ ϴ ˷ ֽϴ.

׷ secreated cytokine Ȯ, 񱳴 T cell Ȱ ȭ, pathogenic infection,

chronic inflammation, allergy, autoimmunity  ʼ̶ ֽϴ.

 

   T cell subset Secreted Cytokine

 

Quantikine ELISA

DuoSet ELISA

ó Ȯ ʹٸ >

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       Th1 > Intracellular pathogen, Autoimmunity

secreted cytokine

human

mouse

rat

Quantikine

DuoSet

Quantikine

DuoSet

Quantikine

DuoSet

IFN-gamma

DIF50

DY285

MIF00

DY485

RIF00

DY585

IL-2

D2050

DY202

M2000

DY402

R2000

DY502

TNF-alpha

DTA00C

DY210

MTA00B

DY410

RTA00

DY510

 

       Th2 > Extracellular bacteria, Allergy & Asthma

secreted cytokine

human

mouse

rat

Quantikine

DuoSet

Quantikine

DuoSet

Quantikine

DuoSet

IL-4

D4050

DY204

M4000B

DY404

R4000

DY504

IL-5

D5000B

DY205

M5000

DY405

-

-

IL-9

-

DY209-05

-

DY409

-

-

IL-13

D1300B

DY213

M1300CB

DY413

-

-

IL-17E

-

-

-

DY1399

-

-

 

       Th17 > Extracellular bacteria & fungi, Autoimmunity

secreted cytokine

human

mouse

rat

Quantikine

DuoSet

Quantikine

DuoSet

Quantikine

DuoSet

IL-17A

D1700

DY317

M1700

DY421

-

-

IL-17F

-

DY1335

M17F0

DY2057

M17F0

DY4437

IL-21

-

-

-

DY594

-

-

IL-22

D2200

DY782

M2200

DY582

M2200

-

TNF-alpha

DTA00C

DY210

MTA00B

DY410

RTA00

DY510

IL-6

D6050

DY206

M6000B

DY406

R6000B

DY506

IL-9

-

DY209-05

-

DY409

-

-

 

         Treg > Immune tolerance, Lymphocyte homeostasis, Autoimmunity

secreted cytokine

human

mouse

rat

Quantikine

DuoSet

Quantikine

DuoSet

Quantikine

DuoSet

TGF-beta 1

DB100B

DY240

MB100B

DY1679

MB100B

-

IL-9

-

DY209-05

-

DY409

-

-

IL-10

D1000B

DY217B

M1000B

DY417

R1000

DY522

        Th9, Th22, Tfh, Tfr > ȮϷ

 

   T cell Total solution!!

    Secreted cytokine characterization screening ǰ

    > Proteom profiler Antibody Array,  R&D Luminex Assay

    PBMC, splenocyte CD4 T cell isolation > MagCellect Cell Isolation Kit

     CD4 T cell Differentiation  > Differentiation Cytokine,

                                              CellXVivo Cell Differentiation Kit

     Flow cytometry antibody > Multi-Color Flow Cytometry Kit,

                                         Antibody (Cytokine, Cell surface, Transcription factor)

 

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