Article│Open Access│Published: 23 July 2021

Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells

Mi-Ok Lee, Su-gi Lee, Cho-Rok Jung, Ye Seul Son, Jae-Woon Ryu, Kwang Bo Jung, Jun-Ho Ahn, Jung-Hwa Oh, Hyang-Ae Lee, Jung Hwa Lim, Janghwan Kim, Insu Jang, Jinhyuk Choi, Jaeeun Jung, Kunhyang Park, Byungwook Lee, Dae-Soo Kim, Mi-Young Son & Hyun-Soo Cho

Nature Communications volume 12, Article number: 4492 (2021)

Abstract

Human pluripotent stem cell (hPSC)-derived organoids and cells have similar characteristics to human organs and tissues. Thus, in vitro human organoids and cells serve as a superior alternative to conventional cell lines and animal models in drug development and regenerative medicine. For a simple and reproducible analysis of the quality of organoids and cells to compensate for the shortcomings of existing experimental validation studies, a quantitative evaluation method should be developed. Here, using the GTEx database, we construct a quantitative calculation system to assess similarity to the human organs. To evaluate our system, we generate hPSC-derived organoids and cells, and detected organ similarity. To facilitate the access of our system by researchers, we develop a web-based user interface presenting similarity to the appropriate organs as percentages. Thus, this program could provide valuable information for the generation of high-quality organoids and cells and a strategy to guide proper lineage-oriented differentiation.

논문 보러 가기

성공적인 Organoid Culture의 첫걸음, 좋은 Recombinant Protein을 선택하는 것!

왜! R&D Systems Protein을 사용해야 할까요?

1. More Active than Competitor: 경쟁사 대비 높은 activity를 보입니다!


Recombinant Human Noggin (Catalog # 6057-NG; orange line) and competitor (Blue line)	Recombinant Human R-Spondin 1 (Catalog # 4645-RS; blue line) and competitor (purple line)	Recombinant Human Wnt-3a (Catalog # 5036-WN; orange line) and competitor (blue line)

2. Lot-to-Lot Consistency

Comparison of the bioactivity of three different lots of R&D Systems Recombinant Human R-Spondin 1 demonstrates lot-to-lot consistency in the protein produced from three different manufacturing runs.

Recombinant Human R-Spondin 1

(Catalog # 4645-RS)

Recombinant Human Wnt-3a

(Catalog # 5036-WN)

Organoid protein list

Activin A	BMP-2	BMP-4	BMP-7	EGF	FGF-basic
FGF-7	FGF-9	FGF-10	FGF-19	HGF	Jagged-1
Noggin	R-Spondin 1	R-Spondin 3	Wnt-3a	Wnt-10b