New Application Note

Xeno-free Organoid Generation Workflow From Stem Cell Spheroids Using VitroGel® STEM and VitroGel® ORGANOID Hydrogel System

Start from stem cells directly from liquid nitrogen (skipping the 2D culture process) to establish high-quality 3D spheroids for differentiation to further formation and maturation into organoids. The entire process is animal-origin free! Discover how the xeno-free VitroGel STEM and VitroGel ORGANOID hydrogels are used to generate and culture mid/hind-gut organoids from hPSC stem cells directly from liquid nitrogen.



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VitroGel® STEM



- Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
- High-quality 3D stem cells for differentiation and organoid formation
- Easy scale-up (from laboratory scale to industrial scale)
- Efficient cell harvesting or subculturing


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- Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture, and PDX resources

- Scalable protocol for lab automation

- Easy 20-minute cell harvesting (enzyme-free)


Learn more about VitroGel ORGANOID


Explore the full potential of 3D cell culture research

VITROGEL® hydrogels closely mimic the natural extracellular matrix (ECM) environment and give an outstanding balance of biological functions and operating ease to establish a robust 3D cell culture platform.


Our cutting-edge hydrogels enable high-throughput processing and scale-up of the 3D cell platform for multiple applications, from organoids to stem cells to invasion assay, angiogenesis assay, and more.


Learn more about VitroGel

Click to read more Application Notes, Research Highlights, and Publications on the VitroGel system

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