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This month we¡¯re talking Wnt3a! Wnt3a is a vital signalling protein for embryonic development, with roles in cell fate and patterning. Working in synergy with Activin A, Wnt3a is essential to the generation of stem cell-derived hepatocytes. With high quality liver tissue samples in short supply, these cells can be used to culture liver organoids, enabling disease research and drug toxicity studies in a 3D model, and offering endless regenerative medicine opportunities.
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Human Liver
Organoid Protocol
 
Need a tried and tested method for generating an in vitro liver model for regeneration studies, disease modelling or drug testing? This protocol includes all steps necessary for culturing and passaging human liver organoids, along with tips for differentiation and cryopreservation.
 
 
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Publication Spotlight: Functional Human Gastrointestinal Organoids
 
In this Cell paper, PSCs were differentiated to produce an organoid model containing the three primary germ layers. These components can be utilized for tissue engineering generating complex 3D cultures, including gastrointestinal organoids.
 
 
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Assess Gene Expression Heterogeneity in Hepatocytes
 
Examine Wnt3a gene expression and other targets in the liver with both molecular and morphological context using RNAscope¢â in situ hybridization spatial technology.