Dynabeads Q&A


 

1. Dynabeads Dynamag

 

2. Protein A/G beads IP, Co-IP

 

3. Streptavidin beads

 

 

Co-IP kit home

 

Additional Information

Troubleshooting guide

Tech Tip


Dynabesd Dynamag


 

Q. Dynabeads ?

 

A. Dynabeads Invetrogen õǰ ִ magnetic beadԴϴ. Ų polystyrene Ǿ, beads ο gamma-Fe2O3 Fe3O4 ̷ ֽϴ. Dynabeads supeparamagnetic beaḏ beads ڱ ʽϴ. magnetic material ѷ οֱ target molecule ڼ ʰ ˴ϴ.

 

 

Q. Dynamag ڼ ΰ?

 

A. DynaMag ڼ ڼ NeodymiumڼԴϴ. ڼ ϱ bead ª ð ȿ ð target molecule ū purification ְ ݴϴ.

 

 

Q. Dynamag ֳ?

 

A. 1.5ml tubeδ DynaMag-2 (12321D) DynaMag-spin (12320D) blood collection tube flow tube DynaMag-5, 15ml tube DynaMag-15, 50ml tube DynaMag-50 ֽϴ.

 

 

Q. DynaMag-2 DynaMag-spin ?

 

A. DynaMag-2 DynaMag-spin ǰ 1.5ml tube̶ ֽϴ. ׷ DynaMag-2 16, DynaMag-spin 6 tube ٴ ֽϴ. DynaMag-2 Ϲ sample lockó ٴڿ tube Ǵ Ϳ ؼ DynaMag-spin tube ̱ sample Ͻ ȿ̶ ֽϴ.

 

 


Protein A/G beads IP, Co-IP


 

Q. Dynabeads Protein A Dynabeads Protein G ?

 

A. Dynabeads Protein G Dynabeads protein A protein G recombinant protein A Ǿִ 2.8 micron beadsԴϴ. Protein G A ٸ subclass immunoglobulin ̸ Դϴ. ڼ selection guide ϼ (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Protein-Expression-and-Analysis/Protein-Sample-Preparation-and-Protein-Purification/ProteinSPProteinIso-Misc/Protein-Isolation/Immunoprecipitation-using-Dynabeads-Protein-A-or-Protein-G.html)

 

 

Q. Dynabeads IP ֽϱ?

 

A. IP մϴ. Antibody coupling kit, Co-IP kit, pre-coated in sheep anti-mouse IgG (mouse 1 antibody Բ ), pre-coated in sheep anti-rabbit IgG (rabbit 1 antibody Բ ) ֽϴ. ڼ selection guide ּ. (http://kr.invitrogen.com/site/kr/ko/home/Products-and-Services/Applications/Protein-Expression-and-Analysis/Protein-Sample-Preparation-and-Protein-Purification/ProteinSPProteinIso-Misc/Protein-Isolation/Immunoprecipitation-using-Dynabeads-Protein-A-or-Protein-G.html)

 

 

Q. 4.5 micron large beads IP ֽϱ?

 

A. , մϴ. Dynabeads anti-rat (Cat# 11035) rat origin 1 antibody ̿Ͽ ֽϴ. ׷ beads ǥ б yield Դϴ.

 

 

Q. IP kit Co-IP kit ̴ ?

 

A. kit beads kitο ִ buffer ū ̸ Դϴ.

 

ϴ IP kit Ϲ ˰ ô Dynabeads protein A Ǵ protein G ֽϴ.

ݸ Co-IP kit M-280 Epoxy beads ִµ, M-280 Epoxy beads antibody bindingϱ elution Ŀ antibody beads ʴ´ٴ ֽϴ. ȮϽð ϴ molecule size antibody heavy/light chain size (50kDa/25kDa) Co-IP kit Ͻô ξ մϴ.

Co-IP kit beads activation buffer پ buffer ִ Ϳ ؼ IP Kit antibody binding-washing buffer washing buffer ִٴ Դϴ.

 

 

Q. Kit ִ buffer ֳ?

 

A. Kit ο ִ buffer Ű ƽϴ.

 

 


Streptavidin beads


 

Q. Biotinylated molecule ;

 

A. Dynalbeads ǰ beads ǥ鿡 Streptavidin coating Ǿִ ǰ ֽϴ. biotinylated molecule ôٸ Streptavidin beads Ͻô ϴ. Streptavidin beads beads ũ⳪ Ư¡ beads ֽϴ. Hydrophobic M-280 MyOne T1 beads ַ protein target ϸ hydrophilic M-270 MyOne C1 beads DNA/RNA work մϴ. ڼ Ȩ ּ.

(http://kr.invitrogen.com/site/kr/ko/home/brands/Product-Brand/Dynal/Streptavidin-Coupled-Dynabeads.html?icid=fr-dynabeads-4)

 

 

Q. Biotinylated molecule beads 𸣰ھ

 

A. Dynabeads Streptavidin Trila Kit õ 帳ϴ. ǰ Streptavidin MyOne C1, Myone T1, M-270, M-280 beads 1ml ִ ǰ̱ Ͻ÷ beads ˾ƺ ִٴ ֽϴ.

 

 

Q. ̰ biotinylated DNA .

 

A. Kilobase BINDER kit (60101) ǰ õ 帳ϴ. ǰ 2kb ̻ biotinylated DNA RNA fragment ִ ǰԴϴ. kit M-280 beads Բ binding buffer ִµ, binding buffer ؼ ̰ fragment ȿ capture ֽϴ.

 

 

Q. Kit ִ buffer ֳ?

 

A. Kit ο ִ buffer Ű ƽϴ.

 

 

Q. Biotinylated antibody ̿ؼ cell isolation Ŀ flow-cytometry ϰ ͽϴ.

 

A. CELLection Biotin Binder Kit (Cat# 11533D) ǰ õ 帳ϴ. ش ǰ beads antibody DNA linker DZ DNA liker ִ buffer ̿ؼ beads cell ս и ִ Ư¡ ֽϴ. isolation cell beads bindingǾ־ Ͻ Dynabeads Biotin Binder (Cat# 11047) ǰ Ͻ õ 帳ϴ.


 

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