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2014.07.02  


DuoSet IC ELISA

 

°øµé¿© ½ÇÇèÇߴµ¥, »ó´ë Á¤·® western blot °á°ú¸¸À¸·Î ¸¸Á·ÇÏ½Ç ¼ö ÀÖ³ª¿ä?

¹ø°Å·Î¿î western blot ´ë½Å Æí¸®ÇÑ ELISA ¹æ½ÄÀ¸·Î Á¤·®±îÁö!

 


¿Ö DuoSet IC ELISA kitÀ» »ç¿ëÇØ¾ß ÇÒ±î¿ä?


- ³ôÀº sensitivity!: Western blotº¸´Ù 10~20¹è ³·Àº ³óµµ±îÁö detection °¡´ÉÇÕ´Ï´Ù.

 

- ÀûÀº ¾çÀÇ sample·Î ½ÇÇè °¡´É!: Western blotº¸´Ù 10¹è ÀûÀº ¾çÀÇ sample¸¸ À־ ÃæºÐÈ÷ ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù.

 

- High throughput: ´Ù¾çÇÑ Á¶°Ç¿¡¼­ ¼öÇàÇÑ ½ÇÇèµéÀ»(¼­·Î ´Ù¸¥ cell line, does dependent, ¿©·¯ °¡Áö drugó¸® µî) ÇѲ¨¹ø¿¡ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù! (ÇϳªÀÇ kit´ç 2°³ÀÇ 96-well plate Á¦°ø)

 

- °£´ÜÇÑ ½ÇÇè ¹æ¹ý!: western blotó·³ gelÀ» ¸¸µé°í transfer¸¦ ¼öÇàÇÏ´Â µî º¹ÀâÇÏ°í ¹ø°Å·Î¿î °úÁ¤ ¾øÀÌ, plate¸¦ ÀÌ¿ëÇÑ °£´ÜÇÑ ½ÇÇè ¹æ¹ýÀ» ÅëÇØ ½±°Ô °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.

 

- ªÀº ½ÇÇè ½Ã°£!: ÇÏ·ç°¡ Áö³ª¾ß °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Â western blot¿¡ ºñÇØ ½ÇÇè ´çÀÏ¿¡ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.


ÀÌ·± ºÐµé²² ÃßõÇØ µå¸³´Ï´Ù!


  • Sample ¼ö°¡ ³Ê¹« ¸¹¾Æ¼­ western blotÀ¸·Î È®ÀÎÇϱ⠾î·Á¿ì½Å ºÐµé
  • ¾î¶² drugÀÌ °¡Àå È¿°úÀûÀÎÁö ¾Ë°íÀÚ ÇϽô ºÐµé
  • DrugÀ» ¾î´À ³óµµ·Î ó¸®ÇØ¾ß È¿°ú°¡ ÀÖ´ÂÁö ¾Ë°íÀÚ ÇϽô ºÐµé
  • DrugÀÌ ¼­·Î ´Ù¸¥ cell ŸÀÔ¿¡ µû¶ó¼­ È¿°ú°¡ ´Þ¶óÁö´ÂÁö ¾Ë°íÀÚ ÇϽô ºÐµé
  • ¾î¶² cell lineÀÌ °¡Àå ÁÁÀº modelÀÎÁö ¾Ë°íÀÚ ÇϽô ºÐµé
  • Western blot ÈÄ Á¤·®½ÇÇèÀ» ´Ù½Ã ¼öÇàÇÏ·Á°í °èȹ ÁßÀ̽ŠºÐµé
  • Western blotº¸´Ù ÈξÀ ½¬¿î ½ÇÇè ¹æ¹ýÀ» ã°í °è½Å ºÐµé
  • ´©°¡ ºÁµµ °°Àº Çؼ®À» ÇÒ ¼ö ÀÖ´Â °´°üÀûÀÎ data¸¦ ¾ò°íÀÚ ÇϽô ºÐµé

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DuoSet IC¸¦ ÀÌ¿ëÇÑ ½ÇÇè °á°úµé


Figure 1. Human total Axl DuoSet IC ELISA (#DYC1643)ÀÌ western blot º¸´Ù ÈξÀ ³ôÀº sensitivity¸¦ °¡Áø´Ù´Â °ÍÀ» ÀÔÁõÇÒ ¼ö ÀÖ´Â data. Human glioblastoma cell lineÀÎ A172¸¦ serial dilution ÇÑ ÈÄ western blot°ú human total Axl DuoSet IC ELISA kitÀ» ÀÌ¿ëÇؼ­ ÃøÁ¤ÇÑ °á°ú. DuoSet IC°¡ ÈξÀ ³·Àº ³óµµÀÇ Axlµµ detection ÇÑ´Ù´Â »ç½ÇÀ» È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.


Figure 2. Human Phospho-VEGF R2/KDR DuoSet IC ELISA (#DYC1766)¿Í western blot data ºñ±³. HUVEC cell lins¿¡ recombinant human VEGF 165 100ng/mLÀ» ó¸® ¶Ç´Â ó¸®ÇÏÁö ¾ÊÀº ÈÄ VEGF R2 phosphorylation Á¤µµ¸¦ ºñ±³. DuoSet IC¿¡¼­´Â sample 100ugÀ» »ç¿ëÇß°í western blot¿¡¼­´Â sample 1mgÀ» »ç¿ë. Western blotº¸´Ù 10¹è ÀûÀº ¾çÀÇ sampleÀ» »ç¿ëÇßÀ½¿¡µµ ºÒ±¸ÇÏ°í density´Â DuoSet IC°¡ ÈξÀ ³ô°Ô ³ª¿ÈÀ» È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ °¢ sample »çÀÌÀÇ phosphorylation Á¤µµÀÇ Â÷À̵µ ÈξÀ °´°üÀûÀ¸·Î È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.


DuoSet IC ELISA kit Á¦Ç° º¸·¯ °¡±â >>> Ŭ¸¯!


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  • Capture antibody
  • Detection antibody
  • Standard or recombinent protein as control
  • Streptacidin-HRP

(Kit¸¶´Ù ±¸¼ºÇ°¿¡ Â÷ÀÌ°¡ ÀÖÀ» ¼ö ÀÖÀ¸´Ï ¹Ýµå½Ã kit insert¸¦ Âü°íÇØÁÖ¼¼¿ä!)


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Intracellular moleculeÀ» Á¤·®ÇÒ ¼ö ÀÖ´Â complete ELISA kit

Surveyor IC ELISA kit!

 - ½ÇÇè¿¡ ÇÊ¿äÇÑ ¸ðµç reagentµéÀÌ kit¿¡ Æ÷ÇÔµÈ complete kit

 - º°µµÀÇ À̹ÌÁö Àåºñ ¾øÀÌ ¹Ù·Î Á¤·® °¡´É

 - ÀûÀº ¾çÀÇ sampleµµ detection ÇÏ´Â ³ôÀº sensitivity

Surveyor IC ELISA kit Á¦Ç° º¸·¯ °¡±â >>> Ŭ¸¯!


Á¤·® ½ÇÇè¿¡ ¾Õ¼­ screeningÀ» ¼öÇàÇÏ´Â °Ç ´ç¿¬ÇÑ ¼ø¼­ ¾Æ´Ñ°¡¿ä?

°£ÆíÇÑ screening tool, Proteome Profiler Antibody Array kit!

- Cell signal, pathway °ü·Ã markers¸¦ ÇÑ ÀåÀÇ membrane¿¡¼­ È®ÀÎ!

 - ¼Ò·®ÀÇ sample·Î 100¿©°³ ´Ü¹éÁú µ¿½Ã ºÐ¼®

 - Antibody array ÀÌ¿ë ³í¹® Áß 91%ÀÇ ³í¹®ÀÌ  R&D Proteome Profiler¸¦ ¼±ÅÃ

 - Western blotº¸´Ù ½±°í ºü¸¥ ½ÇÇè¹æ¹ý!

Proteome Profiler¿¡ ´ëÇÑ ´õ ÀÚ¼¼ÇÑ Á¤º¸¸¦ ¿øÇϽøé Ŭ¸¯ÇØÁÖ¼¼¿ä!


Cell lysate °úÁ¤ ¾øÀÌ 2°¡Áö Intracellular proteins µ¿½Ã ºÐ¼®!

Cell-Based ELISA kit!

 - 2°¡Áö Intracellular protein µ¿½Ã ºÐ¼®

   (Phosphorylated & Total protein / Target & House keeping protein)

 - Cell lysis °úÁ¤ ¾øÀÌ ¹Ù·Î assay ½ÃÀÛ

 - º°µµÀÇ Normalization ºÒÇÊ¿ä

 - 10,000 cells/well ·Î assay ÁøÇà °¡´É

Cell-Based ELISA¿¡ ´ëÇÑ ´õ ÀÚ¼¼ÇÑ Á¤º¸¸¦ ¿øÇϽøé Ŭ¸¯ÇØÁÖ¼¼¿ä!

 

Á¦Ç°»ó´ã: Çмú¸¶ÄÉÆÃÆÀ ÇѼҿµ (techserv@woongbee.com / 031-776-3300 ³»¼± 122)