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(Ŭ¸¯Çغ¸¼¼¿ä!)

 

Proteome Profiler

½ÇÇè µ¿¿µ»ó º¸·¯ °¡±â!

 

T cell column

 

 

Luminex Assay

200¿©°¡Áö protein Áß ÃÖ´ë 100°³±îÁö ÀÚÀ¯·Ó°Ô ¼±Åà °¡´É!

 

CCK-8

Cell Viability Assay

CCK-8°ú ÇÔ²² ¿©À¯¸¦ ´©¸®¼¼¿ä.

 

2014.08.20

 


Antibody Array for Apoptosis


Apoptosis´Â ´Ù¾çÇÏ°í º¹ÀâÇÑ signal cascade¸¦ ÅëÇØ ÀϾ°Ô µÇ¸ç ¹ß»ý, ¾Ï, ½Å°æ ÅðÇ༺ Áúȯ µî¿¡¼­ ¸Å¿ì Áß¿äÇÑ ±âÀÛ Áß Çϳª·Î ¾Ë·ÁÁ® ÀÖ½À´Ï´Ù.

¿õºñ¸ÞµðÅØ¿¡¼­´Â Apoptosis °ü·Ã ´Ü¹éÁúµéÀÇ ¹ßÇöÀ» Çѹø¿¡ È®ÀÎÇÒ ¼ö ÀÖ´Â antibody array, apoptosis signalÀÇ key moleculeÀÎ caspaseÀÇ activity assay, apoptosis °ü·Ã ´Ù¾çÇÑ assay kitµîÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù.


Proteome Profiler Antibody Array Kit


Proteome Profiler?

- ¼Ò·®ÀÇ sample·Îµµ ½ÇÇè °¡´É

 

- Apoptosis specific marker 35°³ analyt ¸¦ ÇÑ ÀåÀÇ membrane¿¡ ³Ö¾ú½À´Ï´Ù!

: Intrinsic pathway marker¿Í extrinsic pathway marker¸¦ °ñ°í·ç È®ÀÎÇÒ ¼ö ÀÖÀ½

: Bad, Bax, Bcl-2,  cIAP-1, cIAP-2, Cytochrome c, FADD, Fas µî

: Caspase-3ÀÇ pro form°ú cleaved formÀ» µ¿½Ã¿¡ È®ÀÎ °¡´É!

: p53ÀÇ phosphorylation siteº°·Î ¹ßÇö¾ç Â÷ÀÌ È®ÀÎ °¡´É (S15, S46, S392)

 

- Most referenced array! : Array »ç¿ë ³í¹® Áß 91%°¡ R&D Á¦Ç° »ç¿ë

 

- Western blot Àåºñ¸¸À¸·Îµµ OK!

 

- Western blotº¸´Ù °£ÆíÇÑ ½ÇÇè ¹æ¹ý : ÀÚ¼¼ÇÑ ½ÇÇè ¹æ¹ýÀº µ¿¿µ»óÀ» ÅëÇØ È®ÀÎÇØÁÖ¼¼¿ä!

 

ÀÌ·± ½ÇÇè¿¡ »ç¿ëÇغ¸¼¼¿ä!

- Ç×¾ÏÁ¦, Ç×¾Ï ¹°Áú¿¡ ÀÇÇÑ cancer cell death ±âÀÛ È®ÀÎ

 

- Apoptosis signalingÀ» ÇÑ ´«¿¡ È®ÀÎ

 

- Cell death°¡ ¾î¶² °æ·Î¸¦ ÅëÇØ ÀÌ·ç¾îÁö´ÂÁö È®ÀÎ °¡´É (Intrinsic ¶Ç´Â extrinsic)

 

¸¹Àº ¿¬±¸ÀÚµéÀÌ Apoptosis array kitÀ» ¾î¶² ½ÇÇè¿¡ »ç¿ëÇß´ÂÁö Âü°í³í¹®À» È®ÀÎÇØÁÖ¼¼¿ä!

Âü°í³í¹® º¸·¯ °¡±â

 

»ç¿ë °¡´ÉÇÑ Array Á¦Ç°

ÃßõÁ¦Ç° 1) Human Apoptosis Array Kit (35 analytes, Cat# ARY009)

 

ÃßõÁ¦Ç° 2) Human Cell Stress Array Kit (26 analytes, Cat# ARY018)


°ü·Ã Á¦Ç° ¹× °ü·Ã ÀÚ·á


°ü·Ã Á¦Ç°

1. Cancer ¿¬±¸ÀÇ ÇʼöÇ°! Phospho-array kit

Phopho-RTK, Phospho-MAPK, Phospho-Kinase °ü·Ã markerµéÀ» ÇÑ ÀåÀÇ membrane¿¡¼­ È®ÀÎÇغ¸¼¼¿ä!

2. TUNEL Assays

DNA fragmentationÀº apoptotic cellÀÇ °¡Àå Å« Ư¡ Áß ÇϳªÀ̸ç TUNEL assayÀº DNA fragmenta¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Â °¡Àå º¸ÆíÀûÀÎ ½ÇÇè ¹æ¹ýÀÔ´Ï´Ù.

R&D systems¿¡¼­´Â °í³óµµ·Î Á¤ÀçµÈ TdT enzymeÀ» Æ÷ÇÔÇÑ TUNEL assay kitÀ» Á¦°øÇÏ°í ÀÖÀ¸¸ç, ¼±»ý´Ô²²¼­ ¿øÇϽô ½ÇÇè ¹æ¹ý¿¡ ²À ¸Â´Â ´Ù¾çÇÑ TUNEL assay kitÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. (ÀüÀÚÇö¹Ì°æ ¹æ½Ä, Çü±¤/flow-cytometry ¹æ½Ä, microplate reader, Á¶Á÷ ƯÀÌÀû colorimetric)

3. ApoStat Apoptosis Detection Kit

ApopStat´Â flow cytometry¹æ½ÄÀ» ÀÌ¿ëÇؼ­ apoptotic cell¿¡¼­ÀÇ caspase È°¼º Á¤µµ¸¦ Á¤·®ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù.

4. Annexin V Kits

R&D systems¿¡¼­´Â biotin (for fixed cell), fluorescein (for unfixed cell) µÎ Á¾·ùÀÇ Annexin V Á¦Ç°ÀÌ Ãâ½ÃµÇ°í ÀÖ½À´Ï´Ù.

5. Mitochondrial Membrane Potential Disruption Assay

MitochondriaÀÇ ¸·ÀüÀ§ ºØ±«´Â apoptosis Ãʱ⿡ ³ªÅ¸³ª´Â Çö»óÀÔ´Ï´Ù. TheDePsipher¢â assay kitÀº lipophilic actionÀ» ÀÌ¿ëÇؼ­ mitochondria membrane potential º¯È­¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù.

6. Caspase & Regulators

R&D systems¿¡¼­´Â ELISAÀÇ gold-standardÀÎ Quantikine ELISA kitÀ» ºñ·ÔÇÏ¿© caspase activity assay, ³ôÀº È¿À²À» ÀÚ¶ûÇÏ´Â protein°ú antibody ¹× ´Ù¾çÇÑ Á¾·ùÀÇ caspase inhibitorµéÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. (Pro form, active form ¸ðµÎ ÀÌ¿ë °¡´É)

7. Cell Viability Assay

MTTº¸´Ù ¶Ù¾î³­ °¨µµ, ¼¼Æ÷¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ¹«µ¶¼º, suspension cell¿¡¼­µµ ¿ì¼öÇÑ ÀçÇö¼ºÀ» º¸ÀÌ´Â CCK-8!

8. Additional Apoptosis-related Detection kits and Assays

Apoptosis transcription factor, Bcl-2 family, caspase, Heat shock protein, IAP µî, apoptosis¿Í °ü·ÃµÈ ´Ù¾çÇÑ Á¦Ç°µéÀ» ¼Ò°³ÇØ µå¸³´Ï´Ù.

9. Small Molecules for Apoptosis Research

Cell death¸¦ ÀÏÀ¸Å² ƯÁ¤ pathway¸¦ Á¤ÀÇÇÒ »Ó¸¸ ¾Æ´Ï¶ó apoptosis¸¦ ÀÏÀ¸Å² factor¸¦ Á¤ÀÇÇϱâ À§Çؼ­´Â small moleculeÀ» ÀÌ¿ëÇÑ ½ÇÇèÀÌ Áß¿äÇÕ´Ï´Ù. R&D systemsÀÇ ÀÚȸ»çÀÎ TOCRIS¿¡¼­ Á¦°øÇÏ´Â apoptosis °ü·Ã small moleculeµéÀ» ¸¸³ªº¸¼¼¿ä!

10. Tool for Oxidative Stress

´Ù¾çÇÑ ÁúȯÀÇ ¿øÀÎÀÌ µÇ´Â oxidative stress markerµµ R&D systems ELISA·Î Á¤È®ÇÏ°í °£ÆíÇÏ°Ô ÃøÁ¤Çϼ¼¿ä!

°ü·Ã ÀÚ·á

Apoptosis Signaling Pathway

Apoptosis Catalog

Caspase Activation & Apoptosis

Oxidative Stress Biomarkers

ÀüÈ­ ¶Ç´Â ¸ÞÀÏ·Î ¿¬¶ô Áֽøé ÇÊ¿äÇÑ ÀڷḦ Àü´ÞÇØ µå¸®µµ·Ï ÇÏ°Ú½À´Ï´Ù.

 

Á¦Ç° »ó´ã : Çмú¸¶ÄÉÆÃÆÀ ÇѼҿµ (techserv@woongbee.com/ 031-776-3300 ³»¼±122)