Cell-Based ELISAs

 

Cell lysate 2 Intracellular proteins м!


 

 
  • 2 Intracellular protein м
    • Phosphorylated & Total protein
    • Target & House keeping protein
  • Cell lysis ٷ assay
    • 96 well plate cells seeding & treatment Cells Target abtibody ó
    • Washing buffer & blocking buffer п permeabilized cells ȯ
  • Normalization ʿ
    • Well-to-well variations
  • 10,000 cells/well assay
  • Attached & suspension cells protocol
  • ʿ м (fluorescence) м

 

 

 


 Cell-Based ELISA


 

Step 1

Step 2

Step 3

Step 4

 

 


 Cell-Based ELISA м

 

Figure 1. Human epithelial carminoma cell (A431) EGF R  phosphorylation Ȯ

A431 cells recombinant human EGF (cat # 236-EG) 󵵺 ó , EGF R phosphorylation Human Phospho-EGF R Cell-Based ELISA (cat # KCB1095) . , EGF R phosphorylation normalization ÿ total EGF R level . kit antibodies Western blot

 


 Cell-Based ELISA


 

Description

Cat. No.

Phosphorylation

&

Total

 Phospho-EGF R/ErbB1 (Y1068)  KCB1095
 Phospho-Akt (S473) Pan Specific  KCB887
 Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)  KCB1018
 Phospho-Histone H2AX (S139)  KCB2288
 Phospho-RelA/NFkB p65 (S536)  KCB7226
 Phospho-Tie-2 (Y992)  KCB2720
 Phospho-Smad3 (S423/S425)/Smad2 (S465/S467)  KCB3226
 Phospho-IkB-alpha (S32/S36)  KCB4809
 Phospho-ErbB3/Her3 (Y1262)  KCB5677
 Phospho-STAT3 (Y705)  KCB4607
 Phospho-STAT1 (Y701)  KCB2894
 Phospho-Smad1 (S463/S465)/Smad5 (S463/S465)  KCB7660
 Phospho-p70 S6 Kinase (T389)  KCB8963
 Phospho-PDGF R beta (Y1021)  KCB2316
 Phospho-CREB (S133)  KCB2510
 Phospho-ErbB4/Her4 (Y1188)  KCB4418
 Phospho-FRS2 (Y436)  KCB5126
 Phospho-Akt (T308) Pan Specific  KCB8871
 Phospho-STAT6 (Y641)  KCB3717
 Phospho-STAT5a/b (Y694/Y699)  KCB4190
 Phospho-ErbB2/Her2 (Y1196)  KCB4438
 Phospho-c-Jun (S63)  KCB7499
 Phospho-STAT2 (Y689)  KCB2890
 Rat Phospho-TrkA (Y785)  KCB5479
 Phospho-Bad (S112)  KCB7517
 Phospho-HGF R/c-MET (Y1234/Y1235)  KCB2480
 Phospho-PDGF R beta (Y751)  KCB1767
 Phospho-Progesterone R/NR3C3 (S294)  KCB5955
 Phospho-PRAS40 (T246)  KCB6890
 Phospho-Histone H3 (S10)  KCB7798
 Phospho-p38 MAP Kinase (T180/Y182)  KCB869
 Phospho-VASP (S157)  KCB8019

Target

&

House keeping

 Total HIF-1 alpha

KCB1935

 Total COX-2

KCB4198

 Total iNOS

KCB9502

 Total beta-Catenin

KCB1329

 Cleaved Caspase-8 (Asp391)

KCB705

 Total HO-1/HMOX1

KCB3776

 


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  Cell-Based ELISA Q&A >> Ŭ

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